Background – HeLa cells task: cell passaging, making mediums such as DMEM, aseptic technique usage, cell adhering, hemocytometer cell counting. Usage of inverted microscopes, cell dyes, and fluorescence microscopes.
Long term goals – The ability to use non-lethal chemicals and substances to induce apoptosis via Bcl-2 genes in HeLa cells. Finding and using proper lethal doses to obtain the highest apoptosis rate of the cancer cells without the usage of harmful chemicals.
Background- Using CRISPR cas 9 deletions of viral receptors deleting viral receptors for herpes simplex virus 1 and enterovirus 7. We infected healthy cells from donors in our class with herpes simplex virus to create a personal cell line for our specific usage. Due to Crispr Cas 9 being the mode of entry, we designed our plasmids and forward/reverse primers.
Long-term goals – Using CRISPR gene editing to remove viral receptors on healthy cells to prevent the viruses from attaching to the surface, preventing infection altogether.