Cystic Fibrosis Diagnostic Test Report
PCR data
Below are the PCRs (visualised by agarose gel electrophoresis) to show amplification of partial CFTR transcripts in healthy (H), and CF Patients (P)1-3. Use this data in your report. Analyse the data (both gels) to describe CFTR gene expression. Label appropriate bands on the ladder and verify the amplicon sizes in both gels.
Expected CFTR amplicon was 750 bp. NTC, no template control.
Expected GAPDH amplicon was 600 bp. NTC, no template control.
Image below is the 1 kb ladder, use this as a reference for amplicon sizes: